Analysis Note

Growth promotion test according to ISO 11133 Mixed inoculum of Campylobacter, E.coli and P.mirabilis. Additionaly monoculture of E.coli and P.mirabilis. Afterwards confirmation on mCCDA resp. Tryptic soy agarInoculum on reference medium - Campylobacter coli ATCC 43478 (WDCM 00004): 10 - 100 - Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100 - Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100 - Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000 - Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000 - Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000Growth on mCCDA - Campylobacter coli ATCC 43478 (WDCM 00004): >10 CFU - Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU - Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFUGrowth on Tryptic soy agar - Escherichia coli ATCC 25922 (WDCM 00013): no growth - Escherichia coli ATCC 8739 (WDCM 00012): no growth - Proteus mirabilis ATCC 29906 (WDCM 00023): no growthColony colour - Campylobacter coli ATCC 43478 (WDCM 00004): greyish - Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish - Campylobacter jejuni ATCC 29428 (WDCM 00156): greyishIncubation: Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

Application

Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.

General description

Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.

Linkage

Replaces: 1.00079.0017

Packaging

10 vial in Fibre case